Chloroquine dna intercalation

Discussion in 'Chloroquin' started by ranri, 20-Mar-2020.

  1. winziki Well-Known Member

    Chloroquine dna intercalation


    Note: based on a RGCC chemosensitivity analysis I have seen at a German clinic, Hydroxychloroquine has been effective in killing the cancer cells of 5 out 7 patients that were tested. It is one of very few available drugs that inhibits autophagy, a mechanism associated with its anticancer properties.

    Color perception changes in plaquenil Chloroquine autophagy off target

    Unwinding of the double strands of the DNA helix is a hallmark feature of intercalating drugs, such as chloroquine, ethidium bromide and m-AMSA. As part of characterization of novel agents, it is often important to know whether a novel compound is a DNA intercalator. Oct 01, 2018 The precise mechanism by which Chloroquine exhibits activity is not known. Chloroquine, may exert its effect against Plasmodium species by concentrating in the acid vesicles of the parasite and by inhibiting polymerization of heme. It can also inhibit certain enzymes by its interaction with DNA. Chloroquine has been shown to inhibit RNA and DNA syn- 18-20 thesis in prokaryotes and in Plas- 2, modium knowlesi. Chloroquine, like other intercalators, may work by altering three-dimensional structure. For exam- ple, there is evidence that DNA may form left-handed helices Z-DNAin transcriptionally active regions.

    In cancer, authophagy is the process used by cancer cells to “self-eat” in order to survive. Specifically, if authophagy is prolonged this will become a lethal process to cancer. duing chemotheraphy, radiotheraphy, etc.) authophagy is used by cancer cells to survive. However, note that Chloroquine has other properties as well that may be very well related to anti cancer mechanism, such as zinc ionophore, and others (see below the section on mechanisms).

    Chloroquine dna intercalation

    Protein intercalation in DNA as one of main modes of fixation of the., Chloroquine - FDA prescribing information, side effects and uses

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  4. Dependence of the torsional rigidity of DNA on base composition. Nature 1990, 344 6262, 175-178. DOI 10.1038/344175a0. Pengguang Wu, J. Michael Schurr. Effects of chloroquine on the torsional dynamics and rigidities of linear and supercoiled DNAs at low ionic strength.

    • Interaction of chloroquine with linear and supercoiled..
    • Chloroquine as intercalator a hypothesis revived..
    • Chloroquine as Intercalator a Hypothesis Revived.

    Chloroquine is an intercalating agent that unwinds DNA, resulting in a decrease in negative superhelicity and thus in the negative superhelical free energy activity. We have shown that prodigiosin competes with chloroquine for the unwinding of DNA and prevents its activity, suggesting that binding of prodigiosin to DNA occurs through intercalation between the stacked base pairs of native DNA. Chloroquine could also be acting directly on DNA, altering the expression of Plasmodium genes. Early work on this drug has shown that chloroquine can act as DNA-intercalating agent 19 and this mechanism was used to explain the antimalarial effect of the drug 20. Chloroquine is a member of quinolone family and is a weak intercalating agent. Chloroquine is used for treating amebiasis, rheumatoid arthritis, discoid and systemic lupus erythematosus. Application DNA intercalator. Also used to increase transfection efficiency. Chloroquine diphosphate salt has been used • in in vitro antiplasmodial assays

     
  5. giropa Guest

    Hydroxychloroquine is a quinoline medicine used to treat or prevent malaria, a disease caused by parasites that enter the body through the bite of a mosquito. Taking Plaquenil. Lupus,Fibromyalgia, ana. Treatments. New Plaquenil Guidelines Plaquenil What You Need to Know - Kaleidoscope Fighting Lupus
     
  6. Mr. Third New Member

    Hydroxychloroquine - Side Effects, Dosage, Interactions. Hydroxychloroquine Side Effects Get emergency medical help if you have signs of an allergic reaction hives; difficulty breathing; swelling of your face, lips, tongue, or throat.

    Hydroxychloroquine Uses, Dosage & Side Effects -
     
  7. pavdi User

    How to Interpret LC3 Immunoblotting Autophagy Vol 3, No 6 Microtubule-associated protein light chain 3 LC3 is now widely used to monitor autophagy. One approach is to detect LC3 conversion LC3-I to LC3-II by immunoblot analysis because the amount of L.

    Rapamycin and Chloroquine The In Vitro and In Vivo Effects.