Autophagic flux assay chloroquine

Discussion in 'Chloroquine Phosphate 500 Mg' started by ultrafiremoder, 03-Mar-2020.

  1. rescue New Member

    Autophagic flux assay chloroquine


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    Tenovin-6 impairs autophagy by inhibiting autophagic flux. and examined for LC3B by immunofluorescence assay. Chloroquine or bafilomycin A1, two known inhibitors of autophagic flux, also. The Autophagy LC3 HiBiT Reporter Assay System is a plate-based assay using a luminescent LC3 reporter to quantitatively measure autophagic flux. The easy one-step protocol eliminates wash steps and the need for complex imaging or flow cytometry systems. Chloroquine is a known inhibitor of autophagic flux through the pathway, and chloroquine treatment results in an increase of LC3B puncta in otherwise normal cells. In contrast, siRNA-mediated knockdown of ATG7 expression results in a loss of LC3B puncta after chloroquine treatment Figure 3.

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    Autophagic flux assay chloroquine

    Autophagy Flux Detection Kit StressXpress® SKT-135., Autophagy Assay LC3 Autophagic Flux Assay Promega

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  6. Autophagic flux assay in starved cells with a western blot of LC3. The correct way to calculate autophagic flux based on the LC3-II band is calculated below. Data and densitometric values are hypothetical. Novus Biologicals LC3B antibody was used in this example.

    • Measuring Autophagic Flux with LC3 protein levels, Novus Biologicals.
    • High-Content Analysis Applied To Autophagy Thermo Fisher..
    • Autophagy Assay Kit ab139484 Abcam.

    Therefore, one of the best methods of quantifying autophagy is to measure the autophagic flux. This is how many autophagosomes form and then become degraded. To measure the flux, you need to detect the LC3-II turnover, or the difference in LC3-II levels in the presence versus the absence of lysosomal inhibitors. Annexin V–FITC/PI double-staining assay, the activity and activating cleavages of caspase-3, -8, and -9. MTT assay and colony forming assay were used to assess the effect of matrine on growth and proliferation of HCC cells. Autophagic flux in HCC cells was analyzed using the expression of LC3BI/II and p62/SQSTM1, GFP-LC3 transfection, and Do you have any plans for watching autophagy in your research? The kit includes HRP conjugated antibody for WB of autophagy flux assay. Realize time saving and brief operation! Inhibitors are ready-to-use. Can be used by 1000-fold dilution! Anti-LC3 antibody for immunocytochemistry is contained. Can visualize autophagosomes!

     
  7. Alirina New Member

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  9. Upsss Well-Known Member

    Plaquenil and drinking - Sjogren's World I also take Methotrexate, along with Plaquenil plus many others and I don't drink any alcohol. Then again, this is an individual decision. I would run it by my doctor and see if they thought it was ok. You then could either drink a little wine, or abstain. I have read where a glass of wine OCCASIONALLY wouldn't hurt.

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  10. Hisp User

    Chloroquine and Hydroxychloroquine Toxicity Guidelines. Chloroquine and hydroxychloroquine belong to the quinolone family. They are related drugs with similar clinical indications for use and similar manifestations of retinal toxicity, although their therapeutic and toxic doses differ.

    Chloroquine And Hydroxychloroquine Toxicity - Abstract.